Jeanne C. Stachowiak, David L. Richmond, Thomas H. Li , Françoise Brochard-Wyart, and Daniel A. Fletcher
The challenge of assembling biomolecular systems within lipid bilayer boundaries is fundamental to all cells and is thus an essential feature of cell-like synthetic systems. While many methods for forming vesicles exist, very few have provided a monodisperse population of unilamellar vesicles with unrestricted content. Sandia researchers recently demonstrated a method for simultaneously creating and loading giant unilamellar vesicles by using a pulsed microfluidic jet. Akin to blowing a bubble, the microfluidic jet deforms a planar lipid bilayer into a vesicle that is filled with solution from the jet and separates from the planar bilayer.